Cryopreservation and characterization of canine preantral follicles
نویسندگان
چکیده
منابع مشابه
Vitreous Cryopreservation of Human Preantral Follicles Encapsulated in Alginate Beads with Mini Mesh Cups
To completely avoid ice crystal formation and thus get a higher survival rate, vitrification methods have been commonly used for cryopreservation of oocytes and embryos. However, currently used vitrification methods for oocytes and embryos are not suitable for the cryopreservation of preantral follicles (PFs). In the present study, stainless steel mesh was fabricated into mini mesh cups to vitr...
متن کاملIn vitro culture of goat preantral follicles
Background: The majority of thousands of oocytes in the ovaries are small, non-growing and reside in preantral follicles (PFs). The development of a culture system for preantral follicle may be very useful for understanding the complex mechanism in folliculogenesis at early stages of development as wells as could offer a significant way for the propagation of livestock, including goats. This pa...
متن کاملIn vitro culture of bovine preantral follicles.
Bovine preantral follicles (40-100 microm diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of the four treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; +1%) or FSH plus hypoxanthine (4 mM) in tissue culture medium (TCM 199) suppleme...
متن کاملEffects of different freezing parameters on the morphology and viability of preantral follicles after cryopreservation of doe rabbit ovarian tissue.
OBJECTIVE To compare the effects of dimethyl sulfoxide (DMSO), 1,2-propanediol (PROH), sucrose, trehalose, concentration of cryoprotectants, equilibration method, and postseeding freezing rate on doe rabbit ovarian tissue preservation after freezing, using fractional experimental design. DESIGN Experimental prospective study. SETTING Research institute in veterinary and agronomic colleges. ...
متن کاملI-11: Cryopreservation of Canine Embryos
Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...
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ژورنال
عنوان ژورنال: Cryobiology
سال: 2018
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2018.02.013